¶Àһζ½ºÄÒΪ¶Àһζ¾¼Ó¹¤ÖƳɵĽºÄÒ¡£
2005¡¶ÖйúÒ©µä¡·
¼±Ö§Ìǽ¬º¬Á¿²â¶¨ÏîÏ£º
É«Æ×Ìõ¼þÓëϵͳÊÊÓÃÐÔÊÔÑ飺ÒÔÊ®°ËÍé»ù¹èÍé¼üºÏ¹è½ºÎªÌî³ä¼Á£»ÒÔ¼×´¼-0.4%Á×ËáÈÜÒº£¨50£º50)ΪÁ÷¶¯Ïࣻ¼ì²â²¨³¤Îª350nm¡£ÀíÂÛ°åÊý°´Ä¾Ï¬²ÝËØ·å¼ÆËãÓ¦²»µÍÓÚ1500¡£
¹©ÊÔÆ·ÈÜÒºµÄÖƱ¸£ºÈ¡×Ü»Æͪº¬Á¿²â¶¨Ïîϵı¾Æ·0.15g£¬¾«Ãܳƶ¨£¬ÖÃ25mlÁ¿Æ¿ÖУ¬¼Ó2.5mol/LÑÎËá¼×´¼ÈÜÒº20ml£¬³¬Éù´¦Àí£¨¹¦ÂÊ 250W£¬ÆµÂÊ50kHz)30·ÖÖÓ£¬·ÅÀ䣬ÓÃ2.5mol/LÑÎËá¼×´¼ÈÜҺϡÊÍÖÁ¿Ì¶È£¬»ìÔÈ£¬Â˹ý£¬¾«ÃÜÁ¿È¡ÐøÂËÒº10ml£¬ÖÃ50mlÔ²µ×ÉÕÆ¿ÖУ¬ÓÚ 90¡æˮԡÖмÓÈÈË®½â30·ÖÖÓ£¬·ÅÀ䣬תÒÆÖÁ25mlÁ¿Æ¿ÖУ¬¼Ó¼×´¼ÖÁ¿Ì¶È£¬Ò¡ÔÈ£¬Â˹ý£¬È¡ÐøÂËÒº£¬¼´µÃ¡£
ÎÄÏ×±¨µÀµÄ·½·¨Ò»°ãΪ²â¶¨»ÆͪÀà³É·ÖµÄº¬Á¿£¬È磺
ÂíäìµÈÓÃHPLC·¨²â¶¨
¶Àһζ½ºÄÒÖÐľϬ²ÝËØ¡¢Òì
ÊóÀîËصĺ¬Á¿£¬²ÉÓÃÒÁÀûÌØ-C18É«Æ×Öù( 250mm¡Á4.6mm, 5¦Ìm)£¬Á÷¶¯ÏàΪ¼×´¼-Ë®(50£º50)£¬Á×Ëáµ÷½ÚpHÖµÖÁ3.0£¬¼ì²â²¨³¤360nm£¬Á÷ËÙ0.8 mL/min£¬ÖùÎÂÊÒΡ£ÑùÆ·ÓÃ2.5mol/LÑÎËá¼×´¼»ØÁ÷ÌáÈ¡¡£Ò½Ñ§È«.ÔÚÏß
www.med126.com »ÆÖ¾·¼µÈÓÃRP-HPLC²â¶¨¶Àһζ½ºÄÒÖÐľϬ²ÝËصĺ¬Á¿£¬É«Æ×ÖùΪPhenomenex Luna C18 (2)£¬4.6mm¡Á150mm, 5¦Ìm¡£Á÷¶¯ÏàΪ¼×´¼-0.4%Á×Ëá(50£º50)£¬¼ì²â²¨³¤Îª350nm£¬Á÷ËÙ0.8mL/min£¬ÖùÎÂ35¡æ¡£ÑùÆ·ÓÃ2.5mol/LÑÎËá¼×´¼»ØÁ÷ÌáÈ¡¡£
ÀîïÐǵȲÉÓ÷ֹâ¹â¶È·¨£¬ÒÔ8-ÒÒõ£Ñõ»ù
ɽèÙÜÕ¼×õ¥£¨8-O-acetyl shanzhiside methylester)Ϊ¶ÔÕÕÆ·£¬75%
ÒÒ´¼Îª·´Ó¦ÈܼÁ£¬¼ÓÈë50%ÑÎËáË®½â20min£¬ÒÔ¶Ô¶þ¼×°±»ù±½¼×ȩΪÏÔÉ«¼Á£¬ÔÚ620nm´¦²â¶¨×Ü»·Ï©ÃÑÝÆÜյĺ¬Á¿¡£ÑùÆ·ÓÃÕôÁóË®³¬ÉùÌáÈ¡¡£